We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)

Food images are useful stimuli for the study of cognitive processes as well as eating behavior. To enhance rigor and reproducibility in task-based research, it is advantageous to have stimulus sets that are publicly available and well characterized. Food Folio by Columbia Center for Eating Disorders is a publicly available set of 138 images of Western food items. The set was developed for the study of eating disorders, particularly for use in tasks that capture eating behavior characteristic of these illnesses. (...) It contains foods that are typically eaten, as well as those typically avoided, by individuals with eating disorders. Each image has now been rated across 17 different attributes by a large general United States population sample via Amazon’s Mechanical Turk. Ratings included subjective attributes as well as estimates of nutrient content. Each participant rated a subset of stimulus set food items on all 17 dimensions. Additional description of the image set is provided in terms of physical image information and accurate nutritional information. Correlations between subjective ratings were calculated and an exploratory factor analysis and exploratory cluster analysis completed. Outcomes of the factor analysis suggested foods may be described along three latent factors of healthiness, tastiness, and umami taste; the cluster analysis highlighted five distinct clusters of foods varying on these same dimensions. Descriptive outcomes indicated that the stimulus set includes a range of foods that vary along multiple dimensions and thus is likely to be useful in addressing various research questions surrounding eating behavior and cognition in healthy populations, as well as in those with eating disorders. The provision of comprehensive descriptive information allows for stimulus selection that is optimized for a given research question and promotes strong inference. (shrink)

An abridgement and revision of the author's thesis, University of Edinburgh. Includes bibliographical references.

Cross cultural ethical conflicts are a major challenge for managers of multinational corporations (MNEs) when an MNE''s business practices and a host country''s practices differ. We develop a set of decision principles to help MNE managers deal with these conflicts and illustrate with examples of ethical conflicts faced by MNEs doing business in contemporary Russia (DeGeorge, 1994). We discuss the generalizability of the principles by comparing them to the Donaldson (1989) and Buller and Kohls (1997) decision models. Finally we discuss (...) changes in the cross cultural ethical problems facing MNE managers and offer suggestions for future corporate and academic work on these problems. (shrink)

Cross cultural ethical conflicts are a major challenge for managers of multinational corporations (MNEs) when an MNE's business practices and a host country's practices differ. We develop a set of decision principles to help MNE managers deal with these conflicts and illustrate with examples of ethical conflicts faced by MNEs doing business in contemporary Russia (DeGeorge, 1994). We discuss the generalizability of the principles by comparing them to the Donaldson (1989) and Buller and Kohls (1997) decision models. Finally we discuss (...) changes in the cross cultural ethical problems facing MNE managers and offer suggestions for future corporate and academic work on these problems. (shrink)

This article introduces a novel theoretical framework for psychopathy that bridges dominant affective and cognitive models. According to the proposed impaired integration (II) framework of psychopathic dysfunction, topographical irregularities and abnormalities in neural connectivity in psychopathy hinder the complex process of information integration. Central to the II theory is the notion that psychopathic individuals are “‘wired up’ differently” (Hare, Williamson, & Harpur, 1988, p. 87). Specific theoretical assumptions include decreased functioning of the Salience and Default Mode Networks, normal functioning in (...) executive control networks, and less coordination and flexible switching between networks. Following a review of dominant models of psychopathy, we introduce our II theory as a parsimonious account of behavioral and brain irregularities in psychopathy. The II theory provides a unified theoretical framework for understanding psychopathic dysfunction and integrates principle tenets of affective and cognitive perspectives. Moreover, it accommodates evidence regarding connectivity abnormalities in psychopathy through its network theoretical perspective. (shrink)

Timothy B. Noone - The Light of Thy Countenance: Science and Knowledge of God in the Thirteenth Century - Journal of the History of Philosophy 40:2 Journal of the History of Philosophy 40.2 258-259 Book Review The Light of Thy Countenance: Science and Knowledge of God in the Thirteenth Century Steven P. Marrone. The Light of Thy Countenance: Science and Knowledge of God in the Thirteenth Century. 2 Vols. Leiden: Brill, 2001. Pp. x + 611. Cloth, $90.00. In this, (...) the most complete study of the tradition of divine illumination ever produced in English, Steven Marrone has set the standard for further studies in thirteenth-century epistemology as well as inaugurated a revival in the historiographical significance of philosophical schools for analyzing medieval thought. He conducts his investigation of thirteenth-century epistemology by focusing upon eleven figures seen as belonging.. (shrink)

A large body of research links testosterone and cortisol to male-male competition. Yet, little work has explored acute steroid hormone responses to coalitional, physical competition during middle childhood. Here, we investigate testosterone, dehydroepiandrosterone, androstenedione, and cortisol release among ethnically Chinese boys in Hong Kong, aged 8–11 years, during a soccer match and an intrasquad soccer scrimmage, with 63 participants competing in both treatments. The soccer match and intrasquad soccer scrimmage represented out-group and in-group treatments, respectively. Results revealed that testosterone showed (...) no measurable change. DHEA increased during both treatments in the majority of participants and the degree of change had no relation to independent variables or covariate measures. Most boys experienced androstenedione increases during match play, but no significant differences during the intrasquad soccer scrimmage competitions. The magnitude of change differed significantly between treatments and was positively associated with age. These latter findings suggest boys’ androstenedione responses may be sensitive to competitor type. For most subjects, cortisol significantly increased during match play, decreased during the intrasquad soccer scrimmage, and differed significantly between treatments, suggesting each treatment promoted a different psychological state among competitors. Cortisol/DHEA molar ratio decreased during the intrasquad scrimmage, suggestive of a more relaxed mental state. These data shed new light on potential proximate mechanisms associated with coalitional competition among prepubescent boys, with relevance to adrenarche and life history theory. (shrink)

: This paper examines the way in which climate change's complexity calls forth dialogue on various cross-cultural dimensions which resonate with its multi-dimensional reality. While the IPCC science and the Kyoto Protocol approach this inclusiveness, they ultimately limit the range of voices heard due to the continuation of cultural assumptions that are intertwined with many environmental issues. Following the Earth Charter as an alternative model of cross-cultural dialogue that can inform a methodological approach of climate change, this analysis suggests that (...) a more inclusive sharing can offer a way of attending to limiting assumptions as a means to creating viable regional and global responses. This climatic research etiquette is clarified through focusing upon the continued dominance of economic scarcity and its religious precursor, original sin, in contemporary environmental thought. (shrink)

High school students are at risk for increased sedentary behavior due in part to a decrease in physical activity throughout adolescence and to required sedentary behavior during much of the school day. The purpose of the current study is to examine the impact of using activity workstations in a high school English class for struggling readers. Twenty high school students participated in the study. The participants completed a 16-week study where each participant used an activity workstation for 8 weeks and (...) a traditional desk for 8 weeks in a crossover design for a 40-min period during normal class. They responded to a series of subjective questions about reading and schoolwork at the beginning and end of each 8-week session and followed the READ 180 program designed to help struggling readers during the study. The results indicated that academic performance increased in both desk conditions during the study and from the beginning to the end of the study. In addition, there was a significant improvement in items in the subjective survey related to reading, motivation, and schoolwork in both desk conditions across the study. The current results suggest that using an activity workstation in the classroom did not negatively affect academic performance or students’ perceptions of working on academic assignments compared to the traditional desk condition. These results indicate that activity workstations could be implemented in classrooms to provide students with a non-sedentary option during the school day thus increasing physical activity in students. (shrink)

Ethical issues surrounding the marketing and trade of controversial products such as tobacco require a better understanding. Virginia Slims, an exclusively women’s cigarette brand first launched in 1968 in the USA, was introduced during the mid 1980s to major Asian markets, such as Japan and Korea, dominated by male smokers. By reviewing internal corporate documents, made public from litigation, we examine the marketing strategies used by Philip Morris as they entered new markets such as Japan and Korea and consider the (...) extent that the company attempted to appeal to women in markets where comparatively few women were smokers. The case study of Virginia Slims reveals that the classification of “vulnerable” consumers is variable depending on culture, tobacco firms display responsive efforts and strategies when operating within a “mature” market, and cultural values played a role in informing Philip Morris’ strategic decision to embrace an adaptive marketing approach, particularly when entering the Korean market. Finally, moral questions are raised with tobacco being identified as a priority product for export and international trade agreements being used by corporations, governments, or trade partners in efforts to undermine domestic public health policies. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:LaudatioTimothy B. Noone (bio)On Sunday, July 26, 2009, the Franciscan Institute was pleased to award to Dr. Girard J. Etzkorn its 22nd Franciscan Institute Medal in recognition of a lifetime of scholarship, editing and publication of texts on medieval philosophy and theology, with a special emphasis on the Franciscan intellectual tradition. The ceremony was held in the Trustees Room of Doyle Hall on the campus of St. Bonaventure University (...) and attended by over 100 scholars, students, friends and members of Dr. Etzkorn's extended families. The award ceremony began with a personal appreciation by Dr. Thomas Shannon, Ph.D., emeritus of the Worcester Polytechnic Institute (Worcester, MA), former member during the 1960s with Dr. Etzkorn of the Sacred Heart Province of Friars Minor (St. Louis, MO). It was followed by the formal laudatio of the honorée presented by former colleague and friend, Dr. Timothy B. Noone, Ph.D., of the Catholic University of America, the text of which is printed here. The event concluded with personal remarks by Dr. Etzkorn on his life as a scholar of the Franciscan tradition."Let us praise men of renown and our fathers in their generation." Sirach 44:1The wisdom of Sirach, the last of the Solomonic books of the Old Testament, would seem an appropriate place to begin this evening's festivities. No doubt, Gerry is squirming a bit in his chair, thinking in his customary humility that the words of Sirach are a bit too high for him. But we must learn to acknowledge what is true and great wherever it is, even when (or to put the matter correctly, especially when) the truth and greatness are found in ourselves. So there will be [End Page 259] no holding back on the present occasion, since the text from Sirach points out our path.We should start with a brief sketch of Gerry's life. Born in Kirkwood, Missouri in 1927, Gerry received his primary and secondary education in Missouri before attending the University of Dayton in the fall of 1944. After three semesters studying chemical engineering, he was drafted into the military, serving in the U.S. Army as part of the occupation forces of Japan after the surrender of August, 1945. This seeming irrelevancy actually became quite important in Gerry's life, for it was during his service in Japan that he encountered the Franciscans and decided to enter the Order after returning to the U.S. Once he had completed his degrees in philosophy and theology and been ordained, Gerry was sent to the University of Louvain, where he took his doctorate, producing a thesis that contained a partial edition of Roger Marston's hitherto unpublished Quodlibetal questions.This work at Louvain presaged in many ways the bulk of Gerry's work: he edited and studied a Franciscan medieval text from Latin manuscripts and studied its philosophical and theological doctrines. After returning from Louvain, Gerry taught for ten years at Quincy College and Seminary before leaving the Franciscans in 1971. But the Franciscans and he were far from being through with each other. Within two years Gerry arrived here to work with Fr. Gedeon Gàl on the Ockham Edition, then in the throes of its labors. Initially working as an Associate Editor on the Ockham Edition, Gerry eventually became Chief Editor of the Scotus Project, the successor project at the Institute to the Ockham Edition.During his twenty-two years at the Institute, Gerry kept up a steady pace of editions, some connected with the Institute's research projects and others springing from his own interests. Among the former, we must note three volumes of the Opera theologica of Ockham and four volumes of the Opera philosophica of Scotus; among the latter, we find the complete edition of Roger Marston's Quodlibetal questions, the edition of John Peckham's Quodlibetal questions, numerous articles containing briefer editions of lesser figures, and a volume of manuscript descriptions, analyzing manuscripts never before described in the Vatican Library. [End Page 260]What I have mentioned so far would be enough for any one human being within the scope of one lifetime. But God had more in store for Gerry... (shrink)

In this article, I argue that St. Bonaventure’s account of angelic natural knowledge of singulars is a remote source for the doctrine of intuitive cognition as this doctrine is later articulated in the writings of John Duns Scotus and his contemporaries. The article begins by reminding the reader of the essential elementsof intuitive cognition, then surveys the treatment of angelic knowledge in Bonaventure’s predecessors and contemporaries, and ends with an analysis ofBonaventure’s own teaching. The point on which Bonaventure anticipates Scotus’s (...) teaching is his insistence that angels know truths about singulars by directlycognizing the existence and presence of singulars without receiving any species in the direct cognitive act. (shrink)

Management practitioners and scholars have worked diligently to identify methods for ethical decision making in international contexts. Theoretical frameworks such as Integrative Social Contracts Theory (Donaldson and Dunfee, 1994, Academy of Management Review 19, 252–284) and more recently the Global Business Citizenship Approach [Wood et al., 2006, Global Business Citizenship: A Transformative Framework for Ethics and Sustainable Capitalism. (M. E. Sharpe, Armonk, NY)] have produced innovations in practice. Despite these advances, many managers have difficulty implementing these theoretical concepts in daily (...) practice. Using the example of recent decisions by internet service providers Google, Yahoo, and MSN regarding censorship requirements in China, we offer six heuristic questions to help managers to resolve cross-cultural ethical conflicts in which the firm’s way of doing business differs from the practice in the host country. Recognizing that companies can take different approaches to law and ethics (Paine, 1994, Harvard Business Review 72(2), 107–117), our aim is to provide a management decision process to deal with demands or opportunities for engaging in questionable business practices in a host country. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:Reviewed by:Virtues of the Will: The Transformation of Ethics in the Late Thirteenth Century by Bonnie KentTimothy B. NooneBonnie Kent. Virtues of the Will: The Transformation of Ethics in the Late Thirteenth Century. Washington, D.C.: The Catholic University of America Press, 1995. Pp. viii + 270. Cloth, $44.95.In this admirably written study, Bonnie Kent presents researchers on medieval philosophy with a survey of moral psychology during the crucial period (...) between the death of Thomas Aquinas and the summit of Duns Scotus’s career in the first decade of the fourteenth century. Although there have been many studies of particular issues in particular thinkers during this period and a generous presentation of texts with commentary by the great Dom Lottin (Psychologie et morale aux XIIe et XIIIe siècles, 7 vols. Louvain, 1942–1954), there has not been any lengthy study to date devoted to the period of the late thirteenth century that focused on moral psychology and the will. Simply in drawing attention to this period in the history of moral psychology, Professor Kent has made a tremendous contribution to the study of medieval philosophy, since she has rendered accessible authors that are more often than not neglected and overlooked. Kent, in fact, attempts something more: she places her study in the framework of the general historiography of Scholastic thought and the more recent interest in medieval ethics prompted by those championing virtue ethics.In order to highlight the distinguishing features of recent studies on medieval ethical thought, Kent reviews the established interpretation of philosophy in the thirteenth century. In the opening chapter, entitled “Heroes and Histories,” she points out the features common to the scholarly approaches of Gilson, Van Steenberghen, and even Copleston: (1) a prominent role is assigned to schools and movements, especially Aristotelianism and Augustinianism; (2) metaphysics and epistemology are the primary objects of study along with the relation between philosophy and theology; and (3) the Thomistic point of view is the organizing principle for telling the drama of the period. The last feature means that the history of the period is fundamentally told as streams of influence leading to Aquinas, followed by the golden age of Aquinas and his contemporaries and succeeded by a gradual decline after Aquinas, leading to the demise of Scholastic thought. In the context of the established historiography, accordingly, the approach of Alasdair MacIntyre represents a restatement of the common view, but one more radical in form. Instead of being merely different schools or movements, Augustinianism and Aristotelianism are different worldviews with their own paradigmatic conceptions of rationality, each of which is subsumed into the higher synthesis of Thomism. According to MacIntyre, the passage to modernity is begun by Scotus whose innovative theses in moral psychology and ethics are considered revolutionary.Kent’s stated aim is to challenge the “standard story” found in Gilson and others as well as its revised version in MacIntyre’s writings by providing a detailed study of the [End Page 462] period just prior to Scotus and establishing precisely how revolutionary Scotus’s teachings were when compared to his immediate predecessors. She accomplishes this aim masterfully in a series of chapters dedicated to the sources of medieval thought and elements of moral psychology. Beginning with late thirteenth-century thinkers’ attitude toward Aristotle in her second chapter, she shows in considerable detail that there is little evidence for any serious anti-Aristotelianism in the vast majority of late thirteenth-century authors. Indeed, the posture of these thinkers was to separate Aristotle from radical Aristotelianism and Thomism in order to claim that ancient wisdom was on the side of Christian ethics and psychology. The only exception that Kent finds to this widespread attitude is in the writings of Peter John Olivi whose hostility to pagan philosophy is clearly evident.The focus in the next chapter is on ethical voluntarism, a position characterized by the endorsement of the superiority of will over reason, the claim that human freedom resides primarily in the faculty of the will, that the will can act contrary to reason’s judgment, and that the will, not the intellect, commands the lower powers of the soul. In detailing the advent of... (shrink)

It has been known for many years that the matrix representation of the one-dimensional position-momentum commutator calculated with the position and momentum matrices in a finite basis is not proportional to the diagonal matrix, contrary to what one expects from the continuous-space commutator. This discrepancy has correctly been ascribed to the incompleteness of any finite basis, but without the details of exactly why this happens. Understanding why the discrepancy occurs requires calculating the position, momentum, and commutator matrix elements in the (...) continuous position basis, in which all are generalized functions. The reason for the discrepancy is revealed by replacing the generalized functions with sequences approaching them as their parameter approaches zero. Besides explaining the discrepancy in the discrete and continuous models, this investigation finds an unusual double-peaked sequence for the Dirac delta function. (shrink)